antibody based elisa kits  (R&D Systems)

Journal: Stem Cell Research & Therapy

Article Title: Comparative evaluation of the therapeutic efficacy between human amniotic epithelial cells and human umbilical cord mesenchymal stem cells in premature ovarian insufficiency

doi: 10.1186/s13287-025-04881-7

Figure Lengend Snippet: Characterization of hAECs and hUC-MSCs. A Schematic illustration of the experimental design for comparing the effects of hAECs and hUC-MSCs on ovarian function. B , C Morphological feature and flow cytometric analysis of hAECs and hUC-MSCs. D , E Representative images of double immunofluorescence staining for OCT4 and CK18 or N-cadherin in hAECs and hUC-MSCs. F Multilineage differentiation potential of hUC-MSCs into osteoblasts, adipocytes, and chondrocytes, as assessed by Alizarin Red, Oil Red O, and Alcian Blue staining, respectively. Scale bars represent 25, 50, 100 and 200 μm

Article Snippet: After permeabilization with 0.1% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA), cell were incubated with the following primary antibodies at 4 °C for overnight: OCT4 (1:200, Boster), CK18 (1:200, Boster) and N-cadherin (1:200, Boster).

Techniques: Double Immunofluorescence Staining, Staining